Activation of NFAT-Dependent Gene Expression by Nef: Conservation among Divergent Nef Alleles, Dependence on SH3 Binding and Membrane Association, and Cooperation with Protein Kinase C-θ
- Resource Type
- Authors
- Päivi Huotari; Marita Hiipakka; Aki Manninen; Kalle Saksela; G. Herma Renkema
- Source
- Journal of Virology. 75:3034-3037
- Subject
- Transcriptional Activation
viruses
Immunology
Biology
Transfection
Microbiology
Gene Products, nef
Conserved sequence
src Homology Domains
Jurkat Cells
Virology
Gene expression
Humans
nef Gene Products, Human Immunodeficiency Virus
Nuclear protein
Protein kinase A
Transcription factor
Alleles
Conserved Sequence
Protein Kinase C
Protein kinase C
NFATC Transcription Factors
Cell Membrane
Nuclear Proteins
virus diseases
NFAT
Molecular biology
Genes, nef
Virus-Cell Interactions
DNA-Binding Proteins
Isoenzymes
Protein Kinase C-theta
Insect Science
HIV-1
Simian Immunodeficiency Virus
Transcription Factors
- Language
- ISSN
- 1098-5514
0022-538X
Here we show that the potential to regulate NFAT is a conserved property of different Nef alleles and that Nef residues involved in membrane targeting and SH3 binding are critical for this function. Cotransfection of an activated protein kinase C-θ (PKC-θ) with Nef implicated PKC-θ as a possible physiological cofactor of Nef in promoting NFAT-dependent gene expression and T-cell activation.