Additional file 6: Figure S6. of GLI pathogenesis-related 1 functions as a tumor-suppressor in lung cancer
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- Xiumei Sheng; Bowen, Nathan; Zhengxin Wang
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WDR77 regulates GLIPR1 expression not through the p53 signaling. a Western blot analysis of whole-cell lysates derived from LNCaP cells expressing NT shRNA (lane 1) or WDR77 shRNA (lane 2) with anti-WDR77, −p53, or -actin. b Silencing WDR77 expression decreased the activity of the p53 reporter. LNCaP cells expressing NT or WDR77 shRNA were transfected with 150 ng of the reporter plasmid pGL3-4 × PBE-E4-luc. The transfected cells were allowed to grow for 48 h and then harvested for the luciferase assay (Promega). The values represent the mean ± SD (n = 3). (PDF 431 kb)