Figure S1. Prophylactic autophagosome vaccination did not alter CD3 + CD4+ or CD3 + CD4 + FOXP3+ infiltrates from poly-I:C adjuvant only treatment. Figure S2. Overview of custom 4T1 mutation site peptide array. Figure S3. Vaccinated animals displayed increased IgG signals to 15mers and splenocyte IFNγ recognition of 15mer antigens. Figure S4. Increased IgG signal intensity to 4T1 15mers correlated with IgG signals in naïve animals. Figure S5. Antigens from proteins identified by mass spectrometry were not favored in IgG signal intensity increases. Figure S6. Increased post-vaccination IFNγ secretion in response to 4T1 mutation site peptides. Figure S7. Increased post-vaccination IFNγ secretion upon restimulation with 4T1 tumor cells. (DOCX 1638 kb)