Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I
- Resource Type
- Authors
- Francis Markey; Torgny Persson; Uno Lindberg; Ingrid Blikstad; Lars Carlsson
- Source
- Cell. 15(3)
- Subject
- Blood Platelets
Macromolecular Substances
macromolecular substances
Filamentous actin
Guanidines
General Biochemistry, Genetics and Molecular Biology
HeLa
chemistry.chemical_compound
Structure-Activity Relationship
Magnesium
Actin-binding protein
Lymphocytes
Guanidine
Actin
Deoxyribonucleases
biology
Depolymerization
biology.organism_classification
In vitro
Actins
Molecular Weight
Biochemistry
chemistry
biology.protein
Calcium
Deoxyribonuclease I
HeLa Cells
- Language
- ISSN
- 0092-8674
A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.