Effects of ANGPTL3 antisense oligodeoxynucleotides transfection on the cell growths and invasion of human hepatocellular carcinoma cells
- Resource Type
- Authors
- Huanzhou Xue; Huifeng Zhang; Sihai Zhao; Cheng-En Pan; Haibo Yu; Deyu Li; Lei Wang
- Source
- Scopus-Elsevier
- Subject
- Carcinoma, Hepatocellular
p38 mitogen-activated protein kinases
Cell
Down-Regulation
Biology
In Vitro Techniques
Transfection
p38 Mitogen-Activated Protein Kinases
Downregulation and upregulation
medicine
Humans
MTT assay
Neoplasm Invasiveness
Angiopoietin-Like Protein 3
Cell Proliferation
Analysis of Variance
Hepatology
Cell growth
Reverse Transcriptase Polymerase Chain Reaction
Liver Neoplasms
Gastroenterology
General Medicine
medicine.disease
Molecular biology
medicine.anatomical_structure
Angiopoietin-like Proteins
Matrix Metalloproteinase 9
Oligodeoxyribonucleotides
Lipofectamine
Hepatocellular carcinoma
Angiopoietins
- Language
- ISSN
- 0172-6390
Background/aims The aim of this study was to investigate the effects of antisense oligodeoxynucleotides transfection of angiopioetin-like protein 3 (ANGPTL3) on the proliferation and invasion capacities of human hepatocellular carcinoma SMMC-7721 cells in vitro. Methodology ANGPTL3 antisense oligodeoxynucleotides was transfected into SMMC-7721 cells with Lipofectamine 2000. We used MTT assay to evaluate cell growth. ANGPTL3, p38 mitogen-activated protein kinases (MAPKs) and Matrix metalloproteinase-9 (MMP-9) mRNA expression levels were evaluated by RT-PCR. The invasive potency of SMMC-7721 cells was measured by the transwell cell invasion assay. Results ANGPTL3 antisense oligodeoxynucleotides were successfully transfected into SMMC-7721 cells, resulting in the significant inhibition of ANGPTL3, p38MAPK and MMP-9 mRNA expression. Downregulation of ANGPTL3 inhibited cell proliferation and decreased invasion of SMMC-7721 cells. Conclusions ANGPTL3 may be functionally involved in hepatocellular carcinoma cell proliferation and invasion and is a potential target for hepatocellular carcinoma therapy.