Mitochondrial uncoupler carbonyl cyanide m‐chlorophenylhydrazone induces vasorelaxation without involving KATP channel activation in smooth muscle cells of arteries
- Resource Type
- Authors
- Zhang, Yan‐Qiu; Shen, Xin; Xiao, Xiao‐Lin; Liu, Ming‐Yu; Li, Shan‐Liang; Yan, Jie; Jin, Jing; Gao, Jin‐Lai; Zhen, Chang‐Lin; Hu, Nan; Zhang, Xin‐Zi; Tai, Yu; Zhang, Liang‐Shuan; Bai, Yun‐Long; Dong, De‐Li
- Source
- Subject
- Male
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Dose-Response Relationship, Drug
Uncoupling Agents
Myocytes, Smooth Muscle
Arteries
Research Papers
Mitochondria
Rats
Rats, Sprague-Dawley
Vasodilation
Structure-Activity Relationship
KATP Channels
Animals
- Language
- English
The effects and mechanisms of chemical mitochondrial uncouplers on vascular function have never been identified. Here, we characterized the effects of the typical mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) on vascular function in rat mesenteric arteries and aorta and elucidated the potential mechanisms.Isometric tension of mesenteric artery and thoracic aorta was recorded by using a multiwire myograph system. Protein levels were measured by western blot analyses. Cytosolic [CaAcute treatment with CCCP relaxed phenylephrine (PE)- and high KCCCP induces vasorelaxation by a mechanism that does not involve K