Inhibition of Staphylococcus epidermidis biofilm by trimethylsilane plasma coating
- Resource Type
- Authors
- Qingsong Yu; Hongmin Sun; Meng Chen; Andrew C. Ritts; John E. Jones; Yibao Ma
- Source
- Antimicrobial agents and chemotherapy. 56(11)
- Subject
- Plasma Gases
medicine.drug_class
Surface Properties
Antibiotics
engineering.material
Staphylococcal infections
Microbiology
Antibiotic resistance
Coating
Coated Materials, Biocompatible
Staphylococcus epidermidis
Ciprofloxacin
medicine
Alloys
Humans
Pharmacology (medical)
Mechanisms of Action: Physiological Effects
Pharmacology
Titanium
Cross Infection
Microscopy, Confocal
biology
Chemistry
Biofilm
Drug Resistance, Microbial
Prostheses and Implants
biochemical phenomena, metabolism, and nutrition
Silanes
Staphylococcal Infections
medicine.disease
biology.organism_classification
Stainless Steel
Anti-Bacterial Agents
Infectious Diseases
Biofilms
engineering
Bacteria
medicine.drug
- Language
- ISSN
- 1098-6596
Biofilm formation on implantable medical devices is a major impediment to the treatment of nosocomial infections and promotes local progressive tissue destruction. Staphylococcus epidermidis infections are the leading cause of biofilm formation on indwelling devices. Bacteria in biofilms are highly resistant to antibiotic treatment, which in combination with the increasing prevalence of antibiotic resistance among human pathogens further complicates treatment of biofilm-related device infections. We have developed a novel plasma coating technology. Trimethylsilane (TMS) was used as a monomer to coat the surfaces of 316L stainless steel and grade 5 titanium alloy, which are widely used in implantable medical devices. The results of biofilm assays demonstrated that this TMS coating markedly decreased S. epidermidis biofilm formation by inhibiting the attachment of bacterial cells to the TMS-coated surfaces during the early phase of biofilm development. We also discovered that bacterial cells on the TMS-coated surfaces were more susceptible to antibiotic treatment than their counterparts in biofilms on uncoated surfaces. These findings suggested that TMS coating could result in a surface that is resistant to biofilm development and also in a bacterial community that is more sensitive to antibiotic therapy than typical biofilms.