Adsorption of mesenchymal stem cells and cortical neural stem cells on carbon nanotube/polycarbonate urethane
- Resource Type
- Authors
- Jong Youl Kim; Thomas J. Webster; Jong Eun Lee; Yoonmi Nho; Dongwoo Khang
- Source
- Nanomedicine. 5:409-417
- Subject
- Materials science
Cell Survival
Scanning electron microscope
Neurogenesis
Immunocytochemistry
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
Nanotechnology
Carbon nanotube
Development
Urethane
law.invention
Mice
law
Neurosphere
Cell Adhesion
Extracellular
Animals
Humans
General Materials Science
Cells, Cultured
Neurons
Polycarboxylate Cement
Nanotubes, Carbon
Mesenchymal stem cell
Mesenchymal Stem Cells
Neural stem cell
Biophysics
Adsorption
Stem cell
- Language
- ISSN
- 1748-6963
1743-5889
Background & aim: Carbon nanotubes (CNTs) are a promising material for implantation due to the fact that CNTs are conductive and have nanostructured dimensions that mimic the 3D structure of proteins found in extracellular matrices. We have investigated whether the CNTs interact with various types of stem cells and either selectively enhance survival of stem cells or not. Materials & methods: CNTs used in the experiments are aligned with polycarbonate urethane. Experiments were carried out using mesenchymal stem cells (MSCs) and cortical derived neurospheres. Immunocytochemistry and scanning electron microscopic analysis were performed for determining the favorable surface material (either CNT or polycarbonate urethane array) for cell survival. Results: It was demonstrated that the MSCs and the neurosphere of cortex-derived neural stem cells (NSCs) grew on the CNT array and both MSCs and NSCs interacted with the aligned CNTs. The results suggest that CNTs assist in the proliferation of MSCs and aid differentiation of cortex-derived NSCs. Conclusion: CNTs may be a novel biocompatible nanophase material with the potential for aiding neuron differentiation.