The effect of different technical parameters on the Benford pattern as calculated based on cell line-derived gene expression data described as raw counts. If not mentioned otherwise read length was 100Â bp and all reads were used in the analysis. Truncated reads (25 and 50Â bp) and lower coverage (30, 50 and 80Â % out of the total reads) appear in plot titles. The red line indicates the expected Benford distribution, symbol-marked lines are the distribution observed for three replicates. (PDF 17 kb)