Maize root culture as a model system for studying azoxystrobin biotransformation in plants
- Resource Type
- Authors
- Inge S. Fomsgaard; Mohamed Elhiti; Maheswor Gautam
- Source
- Gautam, M, Elhiti, M A A & Fomsgaard, I S 2018, ' Maize root culture as a model system for studying azoxystrobin biotransformation in plants ', Chemosphere, vol. 195, pp. 624-631 . https://doi.org/10.1016/j.chemosphere.2017.12.121
- Subject
- Environmental Engineering
Agrobacterium
Health, Toxicology and Mutagenesis
0211 other engineering and technologies
02 engineering and technology
010501 environmental sciences
01 natural sciences
Plant Roots
Zea mays
chemistry.chemical_compound
Murashige and Skoog medium
Hairy root
Biotransformation
Environmental Chemistry
Xenobiotic
0105 earth and related environmental sciences
021110 strategic, defence & security studies
biology
Public Health, Environmental and Occupational Health
General Medicine
General Chemistry
Metabolism
Plant
Lettuce
biology.organism_classification
Strobilurins
Pollution
Phytoremediation
Horticulture
Biodegradation, Environmental
Pyrimidines
chemistry
Root
Azoxystrobin
Drug metabolism
Model
- Language
- English
Hairy roots induced by Agrobacterium rhizogenes are well established models to study the metabolism of xenobiotics in plants for phytoremediation purposes. However, the model requires special skills and resources for growing and is a time-consuming process. The roots induction process alters the genetic construct of a plant and is known to express genes that are normally absent from the non-transgenic plants. In this study, we propose and establish a non-transgenic maize root model to study xenobiotic metabolism in plants for phytoremediation purpose using azoxystrobin as a xenobiotic compound. Maize roots were grown aseptically in Murashige and Skoog medium for two weeks and were incubated in 100 μM azoxystrobin solution. Azoxystrobin was taken up by the roots to the highest concentration within 15 min of treatment and its phase I metabolites were also detected at the same time. Conjugated metabolites of azoxystrobin were detected and their identities were confirmed by enzymatic and mass spectrometric methods. Further, azoxystrobin metabolites identified in maize root culture were compared against azoxystrobin metabolites in azoxystrobin sprayed lettuce grown in green house. A very close similarity between metabolites identified in maize root culture and lettuce plant was obtained. The results from this study establish that non-transgenic maize roots can be used for xenobiotic metabolism studies instead of genetically transformed hairy roots due to the ease of growing and handling.