Biodiversity patterns of free-living marine nematodes communities were assessed by certain diversity measures in different localities of Sindh and Balochistan viz., Ibrahim Haidry, Korangi Creek, Clifton, Keamari, Sandspit, Hawksbay, Paradise Point, Mubarak Village, Gadani and Sonmiani. A total of eighty nine nematodes were gathered from the studied areas which belong to sixty one nematode genera and twenty six families of five orders. The highest number of nematodes (30) was recorded from Sandspit beach and lowest (15) was from Mubarak village. The studied area exhibited spatial variation in nematode biodiversity patterns. The species diversity indices demonstrate that Sandspit is the most stressed area where as Mubarak Village is the least stressed area among the ten sampling spots.