Plasmid- or Ribonucleoprotein-Mediated CRISPR/Cas Gene Editing in Primary Murine T Cells
- Resource Type
- Authors
- Marianne, Dölz; Romina, Marone; Lukas T, Jeker
- Source
- Methods in molecular biology (Clifton, N.J.). 2285
- Subject
- CD4-Positive T-Lymphocytes
Gene Editing
Primary Cell Culture
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Workflow
Mice
Electroporation
Phenotype
Ribonucleoproteins
Research Design
Animals
CRISPR-Cas Systems
Cells, Cultured
Plasmids
- Language
- ISSN
- 1940-6029
The CRISPR/Cas technology allows for genome editing in primary T cells. We herein describe the activation of primary murine CD4