This study investigates soil aggregate distribution, soil organic carbon (SOC), and total nitrogen (TN) concentrations in total soil and within aggregates under different land uses in Regosols of Ghannouch, southern arid region of Tunisia. From three sites, i.e., native soils (NS), olive-growing soil (OG), and seasonal-crop soil (SC) land use, four depths were sampled (0–10, 10–20, 20–40, and >40 cm) and separated into three aggregate-size classes (macroaggregates >0.250mm; microaggregates 0.250–0.050 mm; and