[Cloning, fusion expression and identification of thioredoxin encoding gene from
- Resource Type
- Authors
- Zhang, Zi-Gang; Chen, Xiao-Mei; Su, Dan-Hua; Liu, Yuan; Fu, Tao; Duan-Mu, Jia-Miao; Wu, Liang; Jiang, Xu-Gan; Chen, Sheng-Xia; Cao, Jian-Ping
- Source
- Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control. 28(3)
- Subject
- Thioredoxins
Recombinant Fusion Proteins
Animals
Gene Expression
Cloning, Molecular
Toxoplasma
Plasmids
- Language
- ISSN
- 1005-6661
To clone and express the thioredoxin (Trx) from RH strain tachyzoites ofTrx fragment was amplified by PCR and cloned into the pET-28a (+) vector, and the recombinant protein was induced with IPTG and purified by Ni-NTA affinity chromatography. The polyclonal antibody specificity was detected by Western blotting.The trx gene was amplified fromWestern blotting can detect the specificity of polyclonal anti-Trx antibody, which will facilitate the biological functions of Trx.