Optical volumetric projection for fast 3D imaging through circularly symmetric pupil engineering
- Resource Type
- Authors
- Zachary J. Smith; Bo Cai; Yan Shen; Quan Wen; Xiaomin Zhai; Kaiqin Chu; Zeguan Wang; Ronald X. Xu
- Source
- Biomedical Optics Express. 9:437
- Subject
- Point spread function
Materials science
business.industry
Image quality
Resolution (electron density)
02 engineering and technology
Brain tissue
021001 nanoscience & nanotechnology
01 natural sciences
Article
Atomic and Molecular Physics, and Optics
law.invention
010309 optics
Optics
Optical microscope
Position (vector)
law
0103 physical sciences
Depth of field
0210 nano-technology
business
Projection (set theory)
Biotechnology
- Language
- ISSN
- 2156-7085
Monitoring and manipulating neuronal activities with optical microscopy desires a method where light can be focused or projected over a long axial range so that large brain tissues (100 [Formula: see text] thick) can be simultaneously imaged, and specific brain regions can be optogenetically stimulated without the need for slow optical refocusing. However, the micron-scale resolution required in neuronal imaging yields a depth of field of less than 10 [Formula: see text] in conventional imaging systems. We propose to use a circularly symmetric phase mask to extend the depth of field. A numerical study shows that our method maintains both the peak and the shape of the point spread function vs the axial position better than current methods. Imaging of a 3D bead suspension and sparsely labelled thick brain tissue confirms the feasibility of the system for fast volumetric imaging.