Infiltration of metastatic lymph nodes with PD-1
- Resource Type
- Authors
- Shuo, Wang; Yuguang, Song; Michael A, Morse; Pingping, Sun; Guoliang, Qiao; Xiaoli, Wang; Xinna, Zhou; Amy, Hobeika; Jun, Ren; Herbert Kim, Lyerly
- Source
- Am J Cancer Res
- Subject
- Original Article
- Language
- ISSN
- 2156-6976
Tumor metastases to regional lymph nodes are associated with worse outcome for patients with resected non-small cell lung cancer (NSCLC), but there is a wide variation in survival. We hypothesized that infiltration of tumor-involved lymph nodes with activated effector T cells would impact subsequent outcome. A total of 54 lymph nodes (27 N(+) and 15 N(-) collected from 12 patients with Stage IIB (T(2)N(1)M(0)) and 12 N(-) lymph nodes collected from 10 patients with Stage IIA (T(2)N(0)M(0)) who underwent lymphadenectomy during surgical management of their NSCLC) were analyzed for effector T cells expressing activation markers PD-1 and TIM-3 using the Opal-multiple immunofluorescence assay. The frequency of CD3(+)CD8(+) (P=0.0001), CD3(+)CD8(+)TIM-3(+) (P