The role of regulatory B cells in Echinococcus granulosus-infected mice
- Resource Type
- Authors
- Xinwei Qi; Xiumin Ma; Xuan Zhou; Jiaoyu Shan; Jie Lv; Xuelei Liu; Bin Li; Xuemei Liu; Madinaimu Aibibula; Xuanlin Cai
- Source
- Parasitology Research. 120:1389-1404
- Subject
- General Veterinary
biology
medicine.diagnostic_test
Regulatory B cells
medicine.medical_treatment
Spleen
Immunosuppression
General Medicine
biology.organism_classification
Molecular biology
Flow cytometry
Interleukin 10
Infectious Diseases
medicine.anatomical_structure
Immune system
Insect Science
parasitic diseases
medicine
Parasitology
Secretion
Echinococcus granulosus
- Language
- ISSN
- 1432-1955
0932-0113
To investigate the phenotypic changes of the expression level of regulatory B cells and related molecules during the continuous infection of Echinococcus granulosus (E. granulosus) in mice and its relationship with E. granulosus infection and its immune effect. Experimental group mice were inoculated with protoscoleces suspension via intraperitoneally injection to prepare a mouse model of E. granulosus infection. Flow cytometry was used to detect the expression of regulatory B cells CD1dhiCD5+CD19hi cells and CD1dhiCD5+CD19hi IL-10+ cells in spleen and peripheral blood of mice. The expressions of IL-10 and TGF-β1 in mouse serum were detected via ELISA. The liver pathological changes in mice were observed by HE Moreover, the expressions and distribution of IL-10 and TGF-β1 in mice liver were measured through immunohistochemistry. The ELISA test results showed no significant changes in serum IL-10 and TGF-β1 levels in early infected mice. However, at the middle and late stages of infection, the levels of IL-10 and TGF-β1 in the serum of mice increased significantly (P