[Knockdown of STAT3 inhibits proliferation and migration of HepG2 hepatoma cells induced by IFN1]
- Resource Type
- Authors
- Xiaofang, Li; Yuqi, Wang; Ben, Yan; Peipei, Fang; Chao, Ma; Ning, Xu; Xiaoyan, Fu; Shujuan, Liang
- Source
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 34(2)
- Subject
- STAT3 Transcription Factor
Carcinoma, Hepatocellular
HEK293 Cells
Cell Movement
Gene Knockdown Techniques
Liver Neoplasms
Humans
Interferon-alpha
RNA Interference
Hep G2 Cells
RNA, Small Interfering
Cell Proliferation
Signal Transduction
- Language
- ISSN
- 1007-8738
Objective To prepare lentiviruses expressing shRNA sequences targeting human signal transducer and activator of transcription 3 (STAT3) and detect the effect of STAT3 knockdown on type I interferon (IFN1)-induced proliferation and migration in HepG2 cells. Methods Four STAT3-targeting shRNA sequences (shRNA1-shRNA4) and one control sequence (Ctrl shRNA) were selected and cloned respectively into pLKO.1-sp6-pgk-GFP to construct shRNA-expressing vectors. Along with backbone psPAX2 and pMD2.G vectors, they were separately transfected into HEK293T cells to prepare lentiviruses. HepG2 cells were infected with the lentiviruses. Cytoplastic STAT3 level was detected by Western blotting to screen effective shRNA sequence(s) targeting STAT3. Proliferation and migration of HepG2 cells were analyzed by CCK-8 assay and Transwell