Ex Vivo Phenotype and Frequency of Influenza Virus-Specific CD4 Memory T Cells
- Resource Type
- Authors
- Sharon L. Cunliffe; Andrew Loughry; Paul Klenerman; Wyer; Michaela Lucas; Andrew J. McMichael; Cheryl L. Day
- Source
- Journal of Virology. 78:7284-7287
- Subject
- CD4-Positive T-Lymphocytes
Genes, MHC Class II
Immunology
Orthomyxoviridae
Microbiology
Virus
Virology
Humans
L-Selectin
Pathogen
HLA-DR Antigen
MHC class II
biology
HLA-DR Antigens
T lymphocyte
biology.organism_classification
Phenotype
Cell biology
Insect Science
biology.protein
Pathogenesis and Immunity
Immunologic Memory
Ex vivo
- Language
- ISSN
- 1098-5514
0022-538X
Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct “central memory” CD62L+phenotype.