Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content
- Resource Type
- Authors
- Li Bugao; Zhao Yan; Chang Lu; Cai Chunbo; Yang Yang; Guo Xiaohong; Wei Hei; Gao Pengfei; Wanfeng Zhang; Cao Guoqing; Cheng Zhimin
- Source
- Animal Bioscience
Animal Bioscience, Vol 35, Iss 2, Pp 155-165 (2022)
- Subject
- Adipogenesis
General Veterinary
Physiology
Chemistry
Effector
Adipose tissue
Intramuscular Fat
Nicotinamide Adenine Dinucleotide Phosphate (NADPH)
Lipid metabolism
Transfection
Animal Breeding and Genetics
Lipid Metabolism
Article
Cell biology
chemistry.chemical_compound
QL1-991
Lipid droplet
Adipocyte
Genetics
Animal Science and Zoology
Zoology
Gene
Food Science
Glutamic-oxaloacetic Transaminase 1 (GOT1)
- Language
- English
- ISSN
- 2765-0235
2765-0189
Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p