Cystic fibrosis is a hereditary disease manifested by a change in lung conditions leading to increased colonization of pathogens, especially Pseudomonas aeruginosa (PA). The lung epithelial cells of cystic fibrosis (CF) patients frequently contain glycoconjugates with low sialylation which leads to the reveal of carbohydrate structures to which PA are bind easier, e.g. with PA-IIL lectin. Treatment of PA infection with antibiotics is problematic due to the formation of resistance. Therefore, new therapeutic approaches are studied. One of the studied options is passive immunization with chicken antibodies against PA-IIL lectin. These antibodies show the ability to reduce PA adhesion to lung epithelial cells ex vivo. In order to further study the effect of chicken antibodies, it is necessary to create an appropriate experimental in vivo model. The aim of this study was to optimize the mouse model to mimic lung conditions as in CF patients. First of all, it was necessary to select a suitable bioluminescent strain from these three strains PA-lux 1, PA-lux 2 and PA-XEN 41. On the basis of the highest measured luminescence value, the strain PA-lux 1 cultivated in a conical bottom tube was selected. Subsequently, an experiment was carried out on mice in which the effect of the enzyme neuraminidase and the...