Adjuvantation of inactivated Foot and Mouth Disease Virus vaccine with IL-15 expressing plasmid improves the immune response in Guinea Pigs
- Resource Type
- Authors
- G. R. Reddy; Vijay Nagaraj; Singaram Bharatiraja; H. J. Dechamma; Lijo John
- Source
- Biologicals. 49:23-27
- Subject
- 0301 basic medicine
T-Lymphocytes
medicine.medical_treatment
T cell
Guinea Pigs
Bioengineering
CHO Cells
Applied Microbiology and Biotechnology
Virus
03 medical and health sciences
Cricetulus
0302 clinical medicine
Immune system
Adjuvants, Immunologic
Immunity
medicine
Animals
030212 general & internal medicine
Interleukin-15
Pharmacology
B-Lymphocytes
Immunity, Cellular
General Immunology and Microbiology
biology
Viral Vaccines
General Medicine
Virology
Vaccination
030104 developmental biology
medicine.anatomical_structure
Vaccines, Inactivated
Foot-and-Mouth Disease Virus
Inactivated vaccine
Immunology
biology.protein
Cattle
Antibody
Adjuvant
Plasmids
Biotechnology
- Language
- ISSN
- 1045-1056
Foot and mouth disease is a highly contagious disease affecting cloven footed animals. Vaccination using inactivated virus is followed to control the disease. As the immune response conferred by the inactivated vaccine is short lived, there is a need for an alternate vaccine with increased duration of immunity. Inclusion of adjuvant which enhances B and T cell responses is one of the strategies to increase the duration of immune responses of the vaccine. Interleukin 15 is one such a cytokine which improves the cell mediated immune response and also involved in the maintenance of memory T and B cells. In the present communication, we evaluated the role of bovine IL-15 as an adjuvant to inactivated FMD vaccine in guinea pig model. Animals injected with FMD inactivated vaccine and IL-15 plasmid showed improved levels of neutralizing antibodies which were maintained up to 6 months (as the level of neutralizing antibodies is more >1.5 which is considered to give protection). Increased Th1 and Th2 responses (by measuring the level of IL-4 and IFN- gamma responses) were seen in IL-15 adjuvanted guinea pigs compared to animals injected with inactivated vaccine alone.