Fig. S1. GSEA analysis revealed significant enrichment of gene associated with genomic unstable EWS phenotype (A), cell cycle (B) and NF-κB (C) in SYK knockdown cells, and gene associated with metastasis (D) in cells treated with the SYK inhibitor. Fig. S2. Representative IHC photos of p-SYK expression in TC71 control and shSYK cells. No Ab, no antibody control; NC, scramble shRNA control. Fig. S3. TC71 cells were treated with 4 μmol/L GS-9973 or PRT062607 for 48 h, and cell cycle distribution was analyzed by PI staining. Fig. S4. TC71 and SKES1 cells were infected with control (NC) or sh-MYC lentivirus for 72h, and c-MYC protein level was evaluated by immunoblotting assay. Fig. S5.Total nuclear RNA was immunoprecipitated by the antibody-coated beads (either nonspecific IgG or anti-EZH2 antibody), reverse-transcribed and the cDNA was further amplified by qRT-PCR. Bar graphs displayed the relative MALAT1 levels (Y-axis) in different groups. Protein lysates (input, IgG and anti-EZH2 antibodies) were immunoblotted and probed with anti- EZH2 antibody (lower panel). Data represent mean {plus minus} SD, n=3.