Figure S5. An example of chimeras formed between gm14920 and H2afb2 in some G1 mice produced by crossing founder L74-5 with a wt. female. (a) genes gm14920 and H2afb2 are not amplified with gene-specific primers in pups #6 and #7, respectively but is amplified in a pup #5. (b) amplification with gm14920-Fw and H2afb2-Rev primers shows the chimeric product in pups #6 and #7. (c) A schematic diagram showing that a chimera was formed between gm14920 and H2afb2 genes with a small deletion between these fused genes suggesting that NHEJ mechanisms were involved. Fw, forward primer; Rev., reverse primer; number; L, DNA ladder. (PDF 198 kb)