To study cell response to low dose oxidative stress, radiation adaptive response (RAR) for cell growth and its molecular mechanism were examined using cultured glial cells. Glial cells cultured from Wistar rats were pre-treated with a low dose of X-rays (0.1 Gy) and subsequently exposed to a challenging dose of X-rays (2 Gy). The decrease of cell growth due to challenging dose of irradiation was suppressed by low dose pre-irradiation. RAR was not observed in the presence of the inhibitors of protein kinase C (PKC) or DNA-dependent protein kinase (DNAPK), and in severe combined immunodeficiency (scid) cells or ataxia-telangiectasia mutated (ATM)-knockout cells. Therefore, PKC, DNAPK, and ATM should be involved in RAR. To study the variant expressions of proteins caused by oxidative stress comprehensively, the effect of H2O2-treatment on the expressions of proteins in glial cells using proteomics was examined. The exposure to H2O2 (100 microM) changed the expression of several spots of proteins in glial cells. Spots of proteins were identified by MALDI-TOF mass spectroscopy after digestion by trypsin in gel. As a result, it was suggested that in glial cells the spot of peroxiredoxin 2 was changed by H2O2-treatment.
資料番号: AA0045438003