Immobilization of Lecitase® ultra on recyclable polymer support: application in resolution of trans-methyl (4-methoxyphenyl)glycidate in organic solvents
- Resource Type
- Authors
- Tirunagari Harini; Shalini Basetty; Thenkrishnan Kumaraguru
- Source
- Tetrahedron: Asymmetry. 28:1612-1617
- Subject
- 0106 biological sciences
chemistry.chemical_classification
Immobilized enzyme
010405 organic chemistry
Organic Chemistry
Xylene
Acetaldehyde
Polymer
01 natural sciences
Catalysis
0104 chemical sciences
Inorganic Chemistry
chemistry.chemical_compound
Hydrolysis
Enzyme
Adsorption
chemistry
010608 biotechnology
Organic chemistry
Glutaraldehyde
Physical and Theoretical Chemistry
- Language
- ISSN
- 0957-4166
Lecitase® Ultra was immobilized on epoxy-activated polymer (DILBEAD-VWR) functionalized with polyethyleneimine via adsorption and crosslinking with glutaraldehyde. The resolution of methyl trans -(±)-3-(4-methoxyphenyl) glycidate was carried out in xylene ( e.e. >99%, conversion 50%). The enzyme is not inhibited by the 4-methoxy phenyl acetaldehyde produced during hydrolysis and the immobilized enzyme with 7% moisture content works efficiently in an organic phase. While the immobilized enzyme can be recycled several times, the polymer support can also be recycled after removing the immobilized enzyme by washing with 1 M HCl.