Enumeration of Organohalide Respirers in Municipal Wastewater Anaerobic Digesters
- Resource Type
- Authors
- Tania M. Lozano; Brice A. Fiddler; Russel K. Rahi; Bryan J.K. Smith; Mark J. Krzmarzick; Melissa Anne Boothe
- Source
- Microbiology Insights, Vol 8s2 (2015)
Microbiology Insights, Vol 2015, Iss Suppl. 2, Pp 9-14 (2015)
Microbiology Insights
- Subject
- food.ingredient
triclosan
General Chemical Engineering
Triclocarban
lcsh:QR1-502
Dehalobacter
Bioinformatics
lcsh:Microbiology
Microbiology
chemistry.chemical_compound
Desulfomonile
triclocarban
food
Medicine
Dehalococcoides mccartyi
Desulfitobacterium
Original Research
biology
business.industry
16S ribosomal RNA
biology.organism_classification
Triclosan
chemistry
Wastewater
reductive dehalogenation
business
Bacteria
- Language
- ISSN
- 1178-6361
Organohalide contaminants such as triclosan and triclocarban have been well documented in municipal wastewater treatment plants (WWTPs), but the degradation of these contaminants is not well understood. One possible removal mechanism is organohalide respiration by which bacteria reduce the halogenated compound. The purpose of this study was to determine the abundance of organohalide-respiring bacteria in eight WWTP anaerobic digesters. The obligate organohalide respiring Dehalococcoides mccartyi was the most abundant and averaged 3.3 × 107 copies of 16S rRNA genes per gram, while the Dehalobacter was much lower at 2.6 × 104 copies of 16S rRNA genes per gram. The genus Sulfurospirillum spp. was also detected at 1.0 × 107 copies of 16S rRNA genes per gram. No other known or putatively organohalide-respiring strains in the Dehalococcoidaceae family were found to be present nor were the genera Desulfitobacterium or Desulfomonile.