PDF file - 76K, Supplementary figure 3. T cell Proliferation is decreased after co-culture with MDA-MB-157 breast cancer cells following PTEN knockdown. In addition to MDA-MB-231 cells, PTEN knockdown induced PD-L1 expression in MDA-MB-157 breast cancer cell line also decreases T cell proliferation. (A) Representative histogram of carboxyfluorescein succinimidyl ester (CFSE) assays shows decreased T cell proliferation when PBMC were co-cultured with MDA-MB-157 breast cancer cells transduced with PTEN shRNA in comparison with co-culture with parental MDA-MB-157 cells or MDA-MB-157 cells transduced with non-targeting shRNA. Unstimulated PBMC and stimulated PBMC alone were used as negative controls. CFSE analysis was performed on CD4+ or CD8+ cells. (B) Graphical analysis of CFSE data from a representative experiment performed in triplicate. Significance was determined using an ANOVA test (*P