Magnetic Focus Lateral Flow Sensor for Detection of Cervical Cancer Biomarkers
- Resource Type
- Authors
- Steven T. Wereley; Wen Ren; Joseph Irudayaraj; Sulma I. Mohammed
- Source
- Analytical Chemistry. 91:2876-2884
- Subject
- Protein biomarkers
Instrumentation
Uterine Cervical Neoplasms
Biosensing Techniques
Spectrum Analysis, Raman
010402 general chemistry
01 natural sciences
Signal
Analytical Chemistry
Limit of Detection
Valosin Containing Protein
Biomarkers, Tumor
Humans
Sensitivity (control systems)
Flow sensor
Magnetite Nanoparticles
Reagent Strips
Chemistry
010401 analytical chemistry
Equipment Design
equipment and supplies
0104 chemical sciences
Magnet
Female
Focus (optics)
Antibodies, Immobilized
human activities
Biosensor
Biomedical engineering
- Language
- ISSN
- 1520-6882
0003-2700
We report on a magnetic focus lateral flow biosensor (mLFS) for ultrasensitive detection of protein biomarkers in a practical format. With valosin-containing protein as a target protein, we show that the developed mLFS concept could detect as low as 25 fg/mL with magnetic focus to enhance target capture efficiency to deliver a 106-fold improvement in sensitivity compared to that of conventional lateral flow (LF) systems. The conceptualized strategy utilizes a simple magnet placed beneath the three-dimensional printed LF device to concentrate the targets at the signal zone without any additional instrumentation. In addition, protein mixtures extracted from the tissue of cervical cancer patients was also utilized to validate the sensor. To investigate the effect of magnetic focus on sensitivity, surface-enhanced Raman spectroscopy and dark-field imaging was utilized to characterize the distribution and movement of Fe3O4 core–Au shell nanoprobes in a model LF strip. Our experiments show that the magnetic foc...