Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells
- Resource Type
- Authors
- Michel Aurrand-Lions; Julien M. P. Grenier; Stéphane J. C. Mancini; Marjorie C. Delahaye
- Source
- Bone Marrow Environment
Bone Marrow Environment, 2308, Springer US, pp.73-81, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1425-9_6⟩
Methods in Molecular Biology ISBN: 9781071614242
- Subject
- 0301 basic medicine
medicine.diagnostic_test
[SDV]Life Sciences [q-bio]
Biology
Protein expression
Flow cytometry
Cell biology
03 medical and health sciences
Haematopoiesis
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
medicine
Bone marrow
Progenitor cell
ComputingMilieux_MISCELLANEOUS
030215 immunology
- Language
- English
Flow cytometry has been widely used to detect a single event by means of multiparametric fluorescence measurements. Here we describe a method to analyze subsets of hematopoietic stem and progenitor cells isolated from long bones of mice. We further show that this method allows for comparing JAM-C protein expression between subsets of hematopoietic stem and progenitor cells.