Curcumin inhibits liver cancer by inhibiting DAMP molecule HSP70 and TLR4 signaling
- Resource Type
- Authors
- Shudi Luo; Zhichao Jiang; Biqiong Ren; Fei Xu; Guoying Zou; Tieqiu Yin; Junlong Liu; Xuefei Tian; Yiran Huang
- Source
- Oncology reports. 40(2)
- Subject
- 0301 basic medicine
Cancer Research
Curcumin
Cell
Antineoplastic Agents
Apoptosis
HSP72 Heat-Shock Proteins
S Phase
03 medical and health sciences
chemistry.chemical_compound
medicine
Extracellular
Tumor Microenvironment
Alarmins
Humans
Neoplasm Invasiveness
Neoplasm Metastasis
Cell Proliferation
Oncogene
Chemistry
Liver Neoplasms
General Medicine
Hep G2 Cells
Cell cycle
Hsp70
Toll-Like Receptor 4
030104 developmental biology
medicine.anatomical_structure
Oncology
Cancer research
Intracellular
- Language
- ISSN
- 1791-2431
Curcumin has been revealed to inhibit liver cancer, however, no studies have reported that the mechanism of curcumin's action on liver cancer is related to damage-associated molecular pattern (DAMP) molecules heat shock protein 70 (HSP70) and the toll-like receptor 4 (TLR4) signaling. This study aimed to investigate whether the activation of TLR4 signaling by HSP70 could be inhibited by curcumin, thus investigating the possible mechanism of curcumin in the inhibition of liver cancer. Western blotting was used to evaluate the expression of the HSP70 and TLR4 in HepG2 cells and ELISA was used to detect the concentration of HSP70 in cell culture medium. A thermal tolerance HepG2 (HepG2TT) cell model was established to simulate HSP70 accumulation in the microenvironment. A certain concentration of curcumin was co-cultured with HepG2 and HepG2TT cells to observe the changes of HSP70 and TLR4. Our results revealed that heat stress significantly increased the expression of extracellular HSP70 (eHSP70) and TLR4 (P