The development of diabetes is closely related to the gut microbiota in recent studies, which can be influenced by intestinal motility. A few studies report that electroacupuncture (EA) can lower blood glucose. EA can promote colonic motility and influence gut microbes. In this study, we explored the effect of the EA on blood glucose level in mice with type 2 diabetes (T2D) and its mechanism.The T2D mice model, fecal microbiota transplantation mice model, and KitEA lowered the blood glucose level, altered the gut microbiota, and promoted colonic motility in T2D mice. EA-altered microbiota decreased the blood glucose level and insulin resistance in the antibiotics-treated diabetic mice. EA increased tight junction protein, lowered inflammatory factors, and regulated the IKKβ/NF-κB-JNK-IRS-1-AKT pathway in the liver and muscles. EA could not reduce the blood glucose and regulated gut microbiota in the KitEA promoted intestinal motility to regulate the intestinal flora, thereby reducing the level of systemic inflammation, and ultimately lowering the blood glucose by the IKKβ/NF-κB-JNK-IRS-1-AKT signal pathway.背景: 最近的研究表明, 糖尿病的发展与肠道菌群密切相关, 肠道菌群可能会受到肠道运动的影响。一些文献报道电针(EA)可以降低血糖。 EA可以促进结肠运动并影响肠道微生物。在这项研究中, 我们探讨了 EA 对 2 型糖尿病 (T2D) 小鼠血糖水平的影响及其机制。 方法: 采用T2D小鼠模型、FMT(粪菌移植)小鼠模型和Kit