Additional file 1: Supplementary Figure 1. MiR-669c-3p levels are upregulated in primary murine microglia upon LPS and IFN-γ challenge. LPS or IL-4 exposure alone does not alter BV2 cell (A) or primary microglial cell (B) expression of miR-669c-3p, yet miR-669c-3p expression is induced in primary microglia upon IFN-γ and LPS combined stimulation (M1) (B). Quantitative real-time PCR for miR-669c-3p in wild type BV2 cells treated with vehicle, LPS (50 ng/ml) or IL-4 (20 ng/ml) for 24 h or primary murine microglia treated with vehicle or IFN-γ (20 ng/ml) in combination with LPS (10 ng/ml) for 24 h. One-way ANOVA followed by Bonferroni's post-hoc tests: N = 6-7 in each group. MiR-669c-3p expression is higher in unstimulated primary microglia in comparison to primary astrocytes (C). Quantitative real-time PCR for miR-669c-3p in vehicle-treated primary murine microglia (MG) or vehicle primary murine astrocytes (ASTRO). Unpaired two-tailed t-test: **p < 0.01 compared to vehicle treated astrocytes, N = 3-6. MiR-669c-3p (669) expression is increased in LV1-miR-669c transduced BV2 cells versus LV1-GFP (GFP) transduced cells (D). Quantitative real-time PCR for miR-669c-3p in BV2 cells transduced either with control LV1-GFP or LV1-miR-669c. Unpaired two-tailed t-test: ***p < 0.001 compared to LV1-GFP transduced vehicle, N = 4-6 in each group.