Technical Aspects of Crossmatching in Transplantation
- Resource Type
- Authors
- Daniel S. Ramon; Andrés Jaramillo; Scott T. Stoll
- Source
- Clinics in laboratory medicine. 38(4)
- Subject
- Cytotoxicity test
Lymphocyte
Clinical Biochemistry
Transplants
030230 surgery
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Hyperacute graft rejection
medicine
Humans
Lymphocytes
Transplantation
medicine.diagnostic_test
biology
business.industry
Histocompatibility Testing
Biochemistry (medical)
Flow Cytometry
Complement-dependent cytotoxicity
Histocompatibility
medicine.anatomical_structure
Immunology
biology.protein
Antibody
business
030215 immunology
- Language
- ISSN
- 1557-9832
The presence of antibodies directed against HLA molecules expressed on the donor's cells is one the most important risk factor for serious clinical complications after transplantation. The lymphocyte crossmatch is one of the most important tests available to the laboratory as this assay detects the presence of donor-specific anti-HLA antibodies in potential allograft recipients. Early crossmatch methods used a complement-dependent cytotoxicity test, which was useful for detecting anti-HLA antibodies responsible for hyperacute graft rejection but lacked adequate sensitivity and specificity. Consequently, more sensitive and specific crossmatch methods were developed ultimately leading to the flow cytometry crossmatch as the preferred methodology.