Addiitional file 2: Supplementary Figure1. GRK3 expression in PDX tumors of GAC and paired primary tumor and PC. A. Diagram demonstrates the several patients’ PC cells (malignant ascites cells) into nude mice to generate PDXs (left). Western blot of GRK3 expression of Primary and ascites from PDX tumor generated from GAC patient (right). B. Representative co-immunofluorescent staining images of GRK3 and SOX9 expression on more PC specimen. Scale bars, 25µm. C. Representative co-immunofluorescent staining images of GRK3 and SOX9 expression on paired human primary tumor and PC samples. Scale bars, 25µm. Supplementary Figure 2. Overexpression of GRK3 in GAC cells increased malignant behaviors of GAC. A. Cell viability assay (MTS) was performed in MKN45-GRK3 and MKN45-GFP control cells. Fold increases of cell growth in day 6 and day 8 in media with the indicated FBS % was plotted on y-axis. **P