A gene expressed preferentially in the globular stage of somatic embryogenesis encodes elongation-factor 1alpha in carrot
- Resource Type
- Authors
- Ryoichi Kawahara; Atsushi Komamine; Hiroo Fukuda; Satoshi Sunabori
- Source
- European Journal of Biochemistry. 209:157-162
- Subject
- Base Sequence
Sequence Homology, Amino Acid
Somatic embryogenesis
Molecular Sequence Data
Nucleic acid sequence
Gene Expression
DNA
Plants
Biology
Blotting, Northern
Genes, Plant
Peptide Elongation Factors
Biochemistry
Molecular biology
Homology (biology)
Elongation factor
Peptide Elongation Factor 1
Complementary DNA
Vegetables
Gene expression
Amino Acid Sequence
RNA, Messenger
Gene
Peptide sequence
In Situ Hybridization
- Language
- ISSN
- 1432-1033
0014-2956
We have isolated cDNA of genes that are preferentially expressed during somatic embryogenesis of carrot (Daucus carota L.) by differential screening of globular embryos and cells that are dividing in an unorganized manner. As a result of Northern-blot analysis, one of the genes identified in this way, which we refer to as CEM1, was found to be expressed at high levels in somatic embryos at the globular and heart-shaped stages. In-situ hybridization using globular embryos revealed that the mRNA transcribed from CEM1 was located preferentially in the spherical region of the globular embryo. A homology search using the amino acid sequence deduced from the nucleotide sequence of the CEM1 cDNA revealed that CEM1 encodes the eukaryotic translational elongation-factor 1 alpha.