Use of D140 conditional-knockout cell lines to study chromosomal passenger protein function
- Resource Type
- Authors
- Fant, Xavier; Samejima, Kumilo; Carvalho, Ana; Ogawa, Hiromi; Xu, Zhenjie; Yue, Zuojun; Earnshaw, William C.; Ruchaud, Sandrine
- Source
- Fant, X, Samejima, K, Carvalho, A, Ogawa, H, Xu, Z, Yue, Z, Earnshaw, W C & Ruchaud, S 2010, ' Use of D140 conditional-knockout cell lines to study chromosomal passenger protein function ', Biochemical Society Transactions, vol. 38, pp. 1655-1659 . https://doi.org/10.1042/BST0381655
- Subject
- macromolecular substances
Biochemistry
- Language
- English
The CPC [chromosomal passenger complex INCENP (inner centromere protein) Aurora B kinase survivin and borealin] is implicated in many mitotic processes In the present paper we describe how we generated DT40 conditional knockout cell lines for incenp1 and survivin1 to better understand the role of these CPC subunits in the control of Aurora B kinase activity These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity