Multifunctional Magnetic and Upconverting Nanobeads as Dual Modal Imaging Tools
- Resource Type
- Authors
- Marco Scotto; Bruce E. Cohen; María L. García-Martín; Maria Elena Materia; Preethi Bala Balakrishnan; Manuel Leal; Sahitya Kumar Avugadda; Emory M. Chan; Teresa Pellegrino
- Source
- idUS. Depósito de Investigación de la Universidad de Sevilla
instname
Bioconjugate Chemistry
Bioconjugate chemistry, vol 28, iss 11
- Subject
- Biomedical Engineering
Pharmaceutical Science
Nanoparticle
Bioengineering
Nanotechnology
02 engineering and technology
010402 general chemistry
Multimodal Imaging
01 natural sciences
Fluorescence
Cell Line
Medicinal and Biomolecular Chemistry
Mice
chemistry.chemical_compound
Cell Line, Tumor
Quantum Dots
Microscopy
Animals
Humans
Magnetite Nanoparticles
Skin
Pharmacology
chemistry.chemical_classification
Tumor
Luminescent Agents
Microscopy, Confocal
Nanocomposite
Optical Imaging
Organic Chemistry
Polymer
021001 nanoscience & nanotechnology
equipment and supplies
0104 chemical sciences
Microscopy, Fluorescence
chemistry
Quantum dot
Hela Cells
Confocal
Nanoparticles
Agarose
Magnetic nanoparticles
Biochemistry and Cell Biology
0210 nano-technology
Luminescence
HeLa Cells
Biotechnology
- Language
- English
We report the fabrication of aqueous multimodal imaging nanocomposites based on superparamagnetic nanoparticles (MNPs) and two different sizes of photoluminescent upconverting nanoparticles (UCNPs). The controlled and simultaneous incorporation of both types of nanoparticles (NPs) was obtained by controlling the solvent composition and the addition rate of the destabilizing solvent. The magnetic properties of the MNPs remained unaltered after their encapsulation into the polymeric beads as shown by the T2 relaxivity measurements. The UCNPs maintain photoluminescent properties even when embedded with the MNPs into the polymer bead. Moreover, the light emitted by the magnetic and upconverting nanobeads (MUCNBs) under NIR excitation (λexc = 980 nm) was clearly observed through different thicknesses of agarose gel or through a mouse skin layer. The comparison with magnetic and luminescent nanobeads based on red-emitting quantum dots (QDs) demonstrated that while the QD-based beads show significant autofluorescence background from the skin, the signal obtained by the MUCNBs allows a decrease in this background. In summary, these results indicate that MUCNBs are good magnetic and optical probes for in vivo multimodal imaging sensors. U.S. Department of Energy DE-AC02-05CH11231 AIRC 14527 European Research Council 678109