Real-time high resolution melting PCR for identification of the Swedish variant of Chlamydia trachomatis
- Resource Type
- Authors
- Sylvie Cado; S. Raherison; M. Clerc; Sabine Trombert; Christiane Bébéar; F Normandin; Bertille de Barbeyrac
- Source
- Journal of microbiological methods. 78(1)
- Subject
- Microbiology (medical)
Chlamydia trachomatis
medicine.disease_cause
Microbiology
Polymerase Chain Reaction
Sensitivity and Specificity
High Resolution Melt
law.invention
law
medicine
Humans
Transition Temperature
Chlamydiaceae
Molecular Biology
Polymerase chain reaction
Sweden
biology
Genetic Variation
Time resolution
Chlamydia Infections
biology.organism_classification
Virology
Chlamydiales
Disease transmission
- Language
- ISSN
- 1872-8359
We developed a real-time High Resolution Melting PCR to identify the new Swedish variant of Chlamydia trachomatis ncCT. Of 1191 urogenital specimens C. trachomatis-positive by an omp1 real-time PCR, collected in France in 2007–2008, 1128 gave an interpretable profile corresponding to the wild-type strain; no nvCT was found. This test can be used on selected C. trachomatis-positive samples to monitor the nvCT spread.