[Detection of human parvovirus B19, human bocavirus and human parvovirus 4 infections in blood samples among 95 patients with liver disease in Nanjing by nested PCR]
- Resource Type
- Authors
- Rui, Tong; Wei-Min, Zhou; Xi-Jun, Liu; Yue, Wang; Yong-Liang, Lou; Wen-Jie, Tan
- Source
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology. 27(2)
- Subject
- Adult
Male
Adolescent
Coinfection
Liver Diseases
Middle Aged
Polymerase Chain Reaction
Parvovirus
Human bocavirus
Parvovirus B19, Human
Humans
Female
Viremia
Child
Aged
- Language
- ISSN
- 1003-9279
To analyze the infection of human parvovirus B19, human bocavirus (HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.Nested PCR assays were designed and validated to detect B19, HBoV and PARV4, respectively. The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing. The parvovirus infection was analyzed statistically.The detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed. The frequency of B19 and HBoV were 2/95 (2.1%) and 9/95 (9.5%) in blood samples respectively. No PARV4 was detected. HBoV was detected in 3/5 patients with drug-induced hepatitis.Both B19 and HBoV infection were detected in blood from patients with liver disease.