Left Pulmonary Artery Ligation and Chronic Pulmonary Artery Banding Model for Inducing Right Ventricular—Pulmonary Hypertension in Sheep
- Resource Type
- Authors
- Keith E. Cook; Nancy L. Cardwell; Erika B. Rosenzweig; Rachel Donocoff; Jennifer Talackine; Rei Ukita; Andrew Tumen; Neal M. Foley; John W. Stokes; Yuliya Tipograf; Matthew Bacchetta
- Source
- ASAIO J
- Subject
- Male
medicine.medical_specialty
Hypertension, Pulmonary
Ventricular Dysfunction, Right
Biomedical Engineering
Biophysics
Bioengineering
Pulmonary Artery
030204 cardiovascular system & hematology
Article
Pulmonary artery banding
Biomaterials
03 medical and health sciences
0302 clinical medicine
Afterload
Right ventricular hypertrophy
Internal medicine
Animals
Medicine
Ligation
Sheep
Hypertrophy, Right Ventricular
business.industry
General Medicine
Left pulmonary artery
medicine.disease
Pulmonary hypertension
Disease Models, Animal
medicine.anatomical_structure
030228 respiratory system
Ventricle
Cardiology
business
Destination therapy
- Language
- ISSN
- 1058-2916
Pulmonary hypertension (PH) is a progressive disease that leads to cardiopulmonary dysfunction and right heart failure from pressure and volume overloading of the right ventricle (RV). Mechanical cardiopulmonary support has theoretical promise as a bridge to organ transplant or destination therapy for these patients. Solving the challenges of mechanical cardiopulmonary support for PH and RV failure requires its testing in a physiologically relevant animal model. Previous PH models in large animals have used pulmonary bead embolization, which elicits unpredictable inflammatory responses and has a high mortality rate. We describe a step-by-step guide for inducing pulmonary hypertension and right ventricular hypertrophy (PH-RVH) in sheep by left pulmonary artery (LPA) ligation combined with progressive main pulmonary artery (MPA) banding. This approach provides a controlled method to regulate RV afterload as tolerated by the animal in order to achieve PH-RVH, while reducing acute mortality. This animal model can facilitate evaluation of mechanical support devices for PH and RV failure.