PDF file - 21K, BBIR-z T cells loaded with biotinylated molecules and then washed do not produce IFN�� in response to specific antigen stimulation. Following 45min incubation at 37oC with 1ug/ml of mesothelin specific biotinylated antybodies; P4 Biobody or K1 and control Bio-IgG1 antibody, BBIR-z T cells were washed with PBS and tested against plate-immobilized human mesothelin (105 cells/10ng mesothelin/well). After overnight incubation, culture supernatants were analyzed for human IFN�^ cytokine by ELISA. Concentration of IFN�� is expressed in pg/ml (means �� SEM; n = 3).