Additional file 4 of Multiplex immunofluorescence to measure dynamic changes in tumor-infiltrating lymphocytes and PD-L1 in early-stage breast cancer
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- Sanchez, Katherine; Kim, Isaac; Brie Chun; Pucilowska, Joanna; Redmond, William L.; Urba, Walter J.; Martel, Maritza; Yaping Wu; Campbell, Mary; Zhaoyu Sun; Grunkemeier, Gary; Chang, Shu Ching; Bernard, Brady; Page, David B.
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Additional file 4: Figure S3. mIF antibody validation. Antibody validation was performed using conventional/standard chromogenic stain on human FFPE tonsil tissue. TSA-Opal fluorescent stain was performed on the adjacent slides. Images were acquired with a Vectra 3 Automated Quantitative Pathology Imaging system. FOXP3: forkhead box P3; CK: cytokeratin; PD-L1: programmed death ligand 1.