Tick and tick-borne diseases cause major economic losses to world’s dairy sector. India, being tropical country also faces tremendous challenges from wide arrays of ticks that infest all kind of dairy animals. As we understand that any kind of stresses can disturb the physiological parameters of dairy animals that can impact the animal productivity and over-all performance. In this study therefore, an effort was made to evaluate the physiological responses of tick infested and non-infested native (Sahiwal) and cross bred cattle populations in the central plains area of Uttar Pradesh, India. A total of 3570 animals of indigenous and crossbred cattle from organized and unorganized cattle farm located in and around Lucknow, Uttar Pradesh area was surveyed across seasons (winter, hot summer and hot humid) to assess the prevalence of tick infestation and their effect on physiological responses. The present study revealed that the crossbred cattle were most susceptible to tick infestations. The prevalence of tick infestation was significantly (P