Open housing drives the expression of immune response genes in the nasal mucosa, but not the olfactory bulb
- Resource Type
- Authors
- Nicole Kaiser; Torsten Schöneberg; Ingo Bechmann; Anne Butthof; Vera Lede; Carolin Piotrowski; Matthias H. Tschöp; Petra G. Hirrlinger
- Source
- PLoS ONE, Vol 12, Iss 10, p e0187192 (2017)
PLoS ONE
PLoS ONE 12:e0187192 (2017)
- Subject
- 0301 basic medicine
Pathology
Physiology
Molecular biology
Respiratory System
Gene Expression
lcsh:Medicine
Mucous membrane of nose
Mice
Sequencing techniques
Animal Cells
Gene expression
Medicine and Health Sciences
lcsh:Science
Immune Response
Regulation of gene expression
Multidisciplinary
Gene Ontologies
Brain
RNA sequencing
Genomics
Olfactory Bulb
Body Fluids
Cell biology
Anatomy
Cellular Types
Research Article
medicine.medical_specialty
Immunology
Glial Cells
Cribriform plate
Biology
03 medical and health sciences
Immune system
Olfactory Mucosa
Genetics
medicine
Animals
Gene Regulation
Microglial Cells
Sequence Analysis, RNA
lcsh:R
Biology and Life Sciences
Computational Biology
Cell Biology
Genome Analysis
Olfactory bulb
Bulb
Research and analysis methods
Nasal Mucosa
Molecular biology techniques
030104 developmental biology
lcsh:Q
Nasal administration
- Language
- ISSN
- 1932-6203
Nasal mucosa and olfactory bulb are separated by the cribriform plate which is perforated by olfactory nerves. We have previously demonstrated that the cribriform plate is permissive for T cells and monocytes and that viruses can enter the bulb upon intranasal injection by axonal transportation. Therefore, we hypothesized that nasal mucosa and olfactory bulb are equipped to deal with constant infectious threats. To detect genes involved in this process, we compared gene expression in nasal mucosa and bulb of mice kept under specific pathogen free (SPF) conditions to gene expression of mice kept on non-SPF conditions using RNA deep sequencing. We found massive alterations in the expression of immune-related genes of the nasal mucosa, while the bulb did not respond immunologically. The absence of induction of immune-related genes in the olfactory bulb suggests effective defence mechanisms hindering entrance of environmental pathogens beyond the outer arachnoid layer. The genes detected in this study may include candidates conferring susceptibility to meningitis.