Lipoxin A4 promotes reduction and antibiotic efficacy against Pseudomonas aeruginosa biofilm
- Resource Type
- Authors
- Ana R. Rodriguez; P.Y. Kadiyam Sundarasivarao; Kingsley Yin; J.M. Thornton; Jean Walker; Bernd W. Spur
- Source
- Prostaglandins Other Lipid Mediat
- Subject
- 0301 basic medicine
Imipenem
Physiology
medicine.drug_class
Antibiotics
Virulence
030204 cardiovascular system & hematology
medicine.disease_cause
Biochemistry
Article
Microbiology
03 medical and health sciences
0302 clinical medicine
Ciprofloxacin
medicine
Pathogen
Pharmacology
Pseudomonas aeruginosa
Chemistry
Biofilm
Quorum Sensing
Cell Biology
biochemical phenomena, metabolism, and nutrition
Anti-Bacterial Agents
Lipoxins
Quorum sensing
030104 developmental biology
Biofilms
medicine.drug
- Language
- English
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium commonly found in wound infections and airways of cystic fibrosis patients. P. aeruginosa readily forms biofilms which can reduce the efficacy of antibiotics used to eradicate the pathogen. We have previously shown that a Specialized Pro-resolving Mediator (SPM), Lipoxin A4 (LxA(4)) is a quorum sensing inhibitor which can reduce P. aeruginosa virulence. In this study, we examined the direct actions of LxA(4) and RvD(2) on P. aeruginosa biofilm formation and virulence gene expression. The influence of LxA(4) on antibiotic efficacy and the combined effects on biofilm formation were also investigated. LxA(4) and RvD(2) reduced P. aeruginosa biofilm formation and virulence gene expression. LxA(4) increased ciprofloxacin inhibition on biofilm formation but did not affect ciprofloxacin’s action on non-adherent bacteria. On the other hand, LxA(4) increased bacterial killing action of imipenem but did not affect imipenem’s action on biofilm. We also found that Journal LxA(4) can increase ciprofloxacin’s bacterial killing ability in established biofilm. Together these results suggest that LxA(4) has direct effects on P. aeruginosa biofilm formation and can increase antibiotic efficacy directly.