It is very important for our research that we are able to examine the orientation of packets and their relationship to directions of stress that cause plastic deformation. We use electron backscatter diffraction (EBSD) to achieve this. EBSD examination requires very careful sample preparation. In our work we have developed a sample preparation method for electron bakcscatter diffraction examination. In this study we present the method, which consists of multistage mechanical grinding, polishing and ion polishing. Optimal parameters for each steps (eg.: grinding, polishing and sputtering time, milling angle) were determined for lath martensitic steel, however, it could be used for other type of steel with minor adjustments.