Publisher Correction: A CD146 FACS Protocol Enriches for Luminal Keratin 14/19 Double Positive Human Breast Progenitors
- Resource Type
- Authors
- Ole W. Petersen; Jiyoung Kim; Agla J. Fridriksdottir; Lone Rønnov-Jessen; Mikkel Morsing; Vera Timmermans-Wielenga; Ólöf Gerdur Ísberg; René Villadsen
- Source
- Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-1 (2020)
- Subject
- Adult
Keratin 14
Adolescent
Carcinogenesis
Double negative
lcsh:Medicine
Breast Neoplasms
CD146 Antigen
Young Adult
Medicine
Humans
Progenitor cell
lcsh:Science
Mammary Glands, Human
Cells, Cultured
Keratin-19
Multidisciplinary
business.industry
lcsh:R
Keratin-14
Middle Aged
Publisher Correction
Healthy Volunteers
Cancer research
Neoplastic Stem Cells
CD146
lcsh:Q
Female
business
Human breast
- Language
- English
- ISSN
- 2045-2322
Human breast cancer is believed to arise in luminal progenitors within the normal breast. A subset of these are double positive (DP) for basal and luminal keratins and localizes to a putative stem cell zone within ducts. We here present a new protocol based on a combination of CD146 with CD117 and CD326 which provides an up to thirty fold enrichment of the DP cells. We show by expression profiling, colony formation, and morphogenesis that CD146