Indium inhibits gap junctional communication between rat hepatocytes in primary culture
- Resource Type
- Authors
- Ohno Yasuo; Kawanishi Toru; Guo Xinbiao; Takanaka Akira; Sunouchi Momoko
- Source
- Toxicology letters. 60(1)
- Subject
- Male
chemistry.chemical_element
Tetrazolium Salts
Cell Communication
Biology
Toxicology
Cell junction
Indium
chemistry.chemical_compound
Lactate dehydrogenase
medicine
Animals
Viability assay
Cells, Cultured
Dose-Response Relationship, Drug
L-Lactate Dehydrogenase
digestive, oral, and skin physiology
Gap junction
Rats, Inbred Strains
General Medicine
respiratory system
Molecular biology
Rats
Thiazoles
medicine.anatomical_structure
Biochemistry
chemistry
Liver
Cell culture
Hepatocyte
Toxicity
- Language
- ISSN
- 0378-4274
The effect of indium on gap junctional communication was investigated in primary cultured rat hepatocytes. Treatment of hepatocytes with indium chloride at concentrations of 100 μM to 1 mM for 2 h resulted in dose-dependent inhibition of gap junctional communication between hepatocytes. The effect of indium on hepatocytes was also evaluated using two indices for cell viability: lactate dehydrogenase (LDH) leakage and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. Indium did not cause any increase in LDH leakage from hepatocytes at the above concentrations, but inhibition of MTT reduction was observed at concentrations above 500 μM. These results suggest that the gap junctions between hepatocytes may be vulnerable sites to indium toxicity.