PurposeWe aimed to identify the epithelial-mesenchymal transition and associated putative targets of themiR-34family in transcriptomic data of cervical epithelial squamous cell carcinoma (CESC) to find new therapeutic targets for better disease management.MethodsA combined computational analysis of themiR-34family; gene expression in heterogeneous primary CESCs derived from TCGA; and the integration ofmiR-34band EMT-regulated genes was performed. Four EMT-associatedmiR-34bgene targets were analysed in primary human CESC and non-cancerous cervical tissues by qRT-PCR. Effects of endogenousmiR-34bexpression and its associated gene modulations in cervical cancer cells (C33A and HeLa) were analysed using qRT-PCR, western blotting and immunofluorescence, transwell migration and invasion assays.ResultsThe results showed that themiR-34family might regulate the mTOR pathway, cell cycle (CCND2) and cell adhesion functions (FZD4). Further, we showed that a low negative correlation (r2= −0.07) betweenmiR-34b/EMĨscore and four EMT signature genes (E>MP7, CAV1, ID2, FN1) were significantly regulated bymiR-34b. Also,FN1was indicated as its putative target with the highest negative EMT score and high binding energy between MRE/3’UTR. Further, these transcriptomic signatures in CESC revealed a significant inverse correlation across the stages of primary human CESC. These genes were repressed at transcriptional and translational levels inmiR-34b-3pexpressing C33A and HeLa cells, contributing to their reduced cell migration and invasive properties.ConclusionsOur studies revealed the potential targets of themiR-34family, especiallymiR-34b, that can emerge as potential biomarkers and promising therapeutic targets in CESC disease management.